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Smart Agriculture ›› 2020, Vol. 2 ›› Issue (2): 48-58.doi: 10.12133/j.smartag.2020.2.2.202005-SA004

• Topic--Agricultural Sensor and Internet of Things • Previous Articles     Next Articles

A Fluorescence Based Dissolved Oxygen Sensor

GU Hao1, WANG Zhiqiang1, WU Hao2, JIANG Yongnian3, GUO Ya1()   

  1. 1.School of Internet of Things Engineering, Jiangnan University/Key Laboratory of Advanced Process Control for Light Industry, Ministry of Education, Wuxi 214122, China
    2.Jiangsu Internet Agricultural Development Center, Nanjing 210017, China
    3.Jiangsu Zhongnong Internet of Things Technology Co. , Ltd. , Yixing 214200, China
  • Received:2020-05-12 Revised:2020-06-25 Online:2020-06-30 Published:2020-08-10
  • corresponding author: Ya GUO E-mail:guoy@jiangnan.edu.cn


The measurement of dissolved oxygen content in water is of great significance to aquaculture. However, the dissolved oxygen sensors on the market in China are expensive, and are difficult to maintain continuous online measurement and update parts, so they cannot be widely applied in real production and play expected role in the aquaculture Internet of things(IoT). Based on the principle of fluorescence quenching, a low cost and easy maintenance of dissolved oxygen sensor was developed in this work based on the relationship between the concentration of dissolved oxygen in water and the phase difference of fluorescence signal. The self-made oxygen-sensitive membrane was used to generate red fluorescence which being excited by blue light, and the fluorescence life was regulated by the concentration of dissolved oxygen. Photoelectric conversion circuit with optical signal sensing device was designed to sense optical signal. The STM32F103 microprocessor was used as the main control chip, and the lower computer program was programmed to generate the excitation light pulse. The phase-sensitive detection principle and fast Fourier transform (FFT) were used to calculate the phase difference between the excitation light and the reference light, which was converted into the concentration of dissolved oxygen and realized the measurement of dissolved oxygen. The fluorescence detection part and the main control part of the system were designed as detachable independent modules, and shield lines were used to plug and pull directly, so as to facilitate replacement and maintenance and realize online remote measurement. The testing results showed that, the measurement range of the sensor was 0-20 mg/L, system time delay was less than 2 s, and the life time of the oxygen sensitive membrane would be about 1 year. The dissolved oxygen sensor has the characteristics of convenient measurement, stable result output, low cost and small volume, which will lay a good foundation for the development and marketization of low-cost dissolved oxygen sensors in aquaculture industry of China.

Key words: dissolved oxygen sensor, fluorescence quenching, aquaculture, STM32 microprocessor, oxygen sensitive membrane

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