关键词: 水心病, 高光谱成像技术, X-ray CT成像技术, 可溶性固形物

Abstract:

[Objective] Apple "sugar-glazed core" (also known as watercore) is a common physiological disorder in apple fruits. Apples with watercore possess a distinctive flavor and are highly favored by consumers. However, severely affected apples are prone to mold growth during storage, posing potential food safety risks. Currently, the primary method for detecting sugar-glazed core in apple relies on manual destructive inspection, which is inefficient for large-scale applications and fails to meet the demands of modern automated and intelligent industrial production. To achieve rapid and non-destructive detection of apples with varying watercore severity levels, effective grading and soluble solids content (SSC) prediction models were developed in this study. [Methods] The Xinjiang Aksu Red Fuji apples were used as the research subject. A total of 230 apple samples were selected, comprising 113 normal, 61 mild, 47 moderate, and 9 severe watercore apples. The watercore severity was quantified through image processing of the apples' cross-sectional images. X-ray computed tomography (X-ray CT) data were acquired, and SSC values were measured. A hyperspectral imaging system was used to collect reflectance spectra within the 400~1 000 nm range. After performing black-and-white correction and selecting regions of interest (ROI), the Sample Set Partitioning based on Joint X-Y Distances (SPXY) algorithm was applied to divide the dataset into modeling (training) and prediction sets at a 3:1 ratio. Using the iToolbox in MATLAB, discriminant models were constructed based on partial least squares discriminant analysis (PLS-DA), support vector machine (SVM), and convolutional neural network (CNN) algorithms with reflectance spectral data as the input. Regression models for predicting SSC across different watercore severity levels were also established. Feature wavelength selection was carried out using competitive adaptive reweighted sampling (CARS), successive projections algorithm (SPA), and uninformative variable elimination (UVE) methods. [Results and Discussions] The results indicated that as watercore severity increased, the SSC of Red Fuji apples exhibited an upward trend. The average SSC values were 13.4% for normal apples, 14.9% for mild watercore apples, 15.0% for moderate watercore apples, and 16.0% for severe watercore apples. X-ray CT imaging revealed that the average tissue density of watercore-affected regions was higher than that of healthy tissues. Three-dimensional reconstruction algorithms allowed visualization of the internal spatial distribution of watercore tissues at different severity levels. The spatial volume proportions of watercore tissues were 3.92% in mild, 6.11% in moderate, and 10.23% in severe watercore apples. Apples with severe watercore demonstrated higher spectral reflectance. The PLS-DA-based grading model achieved accuracies of 98.7% in the training set and 95.9% in the test set. The model based on feature wavelengths selected by the UVE algorithm also showed high precision, with accuracies of 95.67% in the training set and 86.06% in the test set. For SSC regression modeling, the partial least squares regression (PLSR) model performed best, with a coefficient of determination for calibration (R_{\mathrm{C}}^{\mathrm{2}}) of 0.962, root mean square error of calibration (RMSEC) of 0.264, coefficient of determination for prediction (R_{\mathrm{P}}^{\mathrm{2}}) of 0.879, and root mean square error of prediction (RMSEP) of 0.435. The model based on feature wavelengths selected by the SPA algorithm exhibited further improved prediction performance, yielding R_{\mathrm{C}}^{\mathrm{2}} 0.846, RMSEC 0.532, R_{\mathrm{P}}^{\mathrm{2}} ^{0.792, RMSEP 0.576, coefficient of determination for cross-validation (R_{\mathrm{C}\mathrm{V}}^{\mathrm{2}}) 0.781, and root mean square error of cross-validation (RMSECV) 0.637. [Conclusions] This study leveraged hyperspectral imaging and X-ray CT technologies to analyze differences in optical reflectance and microstructural characteristics of apple tissues across different watercore severity levels. The developed grading model effectively predicted watercore severity in apples, providing critical technical support for the development of intelligent post-harvest sorting equipment. The SSC regression model accurately predicted SSC values in apples with varying watercore severity, offering an efficient method for non-destructive detection and quality assessment of watercore-affected apples.}

Key words: water core disease, hyperspectral imaging technology, X-ray CT imaging technology, soluble solid content (SSC)