Design and Test of Portable Aflatoxin B1 Detection System

doi:10.12133/j.smartag.SA202303004

Abstract

Abstract:

To achieve rapid on-site detection of aflatoxin B1 (AFB1) in agricultural and sideline products, a portable detection system based on differential pulse voltammetry (DPV) and STM32F103ZET6 as the core processor was designed. The system consists of two main parts: hardware detection devices and a mobile App, which are connected through Wi-Fi communication. The hardware detection equipment includes a DPV waveform generation circuit, constant potential circuit, and micro current detection module. The upper computer App was developed in an Android environment and completed tasks such as signal acquisition and data storage. After completing the design, experiments were conducted to verify the accuracy of the constant potential circuit and micro current detection module. The constant potential circuit accurately applied the voltage set by the program to the electrode, with a maximum error of 4 mV. The micro current detection module converts the current into a voltage signal according to the theoretical formula and amplifies it according to the theoretical amplification factor. The laboratory-made AFB1 sensor was used to effectively detect AFB1 in the range of 0.1 fg/ml to 100 pg/ml. The maximum relative error between the test results in the standard solution and the electrochemical workstation CHI760e was 7.37%. Furthermore, peanut oil samples with different concentrations of AFB1 were tested, and the results were compared to the CHI760e detection results as the standard, with a recovery rate of 96.8%~106.0%. Peanut samples with different degrees of mold were also tested and compared with CHI760e, with a maximum relative error of 7.10%.The system's portability allows it to be easily transported to different locations for on-site testing, making it an ideal solution for testing in remote or rural areas where laboratory facilities may be limited. Furthermore, the use of a mobile App for data acquisition and storage makes it easy to track and manage testing results. In summary, this portable detection system has great potential for widespread application in the rapid on-site detection of AFB1 in agricultural and sideline products.

Key words: AFB1, portable system, STM32, differential pulse voltammetry, constant potential circuit, Wi-Fi

CLC Number:

TP216

WANG Pengfei, GAO Yuanyuan, LI Aixue. Design and Test of Portable Aflatoxin B1 Detection System[J]. Smart Agriculture, 2023, 5(1): 146-154.

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R_RW/kΩ	设定电压值/V
R_RW/kΩ	-2.0	-1.0	0	1.0	2.0
1	-1.998	-0.999	0.001	1.003	2.001
10	-1.996	-0.998	0.002	1.004	2.002
100	-1.998	-0.999	0.000	1.002	2.001
1000	-1.997	-0.996	0.001	1.004	2.000

I_输入/μA	V_理论/V	V_实测/V	绝对误差/V	相对误差/%
10	0.491	0.479	0.012	2.523
20	0.983	1.010	0.026	2.653
40	1.966	2.003	0.036	1.841
60	2.949	2.924	0.025	0.861
90	4.424	4.479	0.054	1.216

浓度/（fg·ml^-1）	CHI760e电流/μA	系统电流/μA	相对误差/%
0	80.34±0.19	80.24±0.23	1.3
0.1	72.71±0.21	69.56±0.22	4.3
100.0	40.66±0.18	42.00±0.24	3.2
100，000.0	3.05±0.17	2.99±0.19	1.8

浓度/（fg·ml^-1）	CHI760e/（fg·ml^-1）	便携式系统/（fg·ml^-1）	相对误差/%
0	0.00±0.00	0.00±0.00	——
1	0.98±0.40	0.91±0.05	7.02
10	10.51±0.03	11.27±0.02	6.72
100	97.30±0.05	90.12±0.06	7.37
1000	989.70±0.02	921.31±0.02	6.91

加入的AFB1浓度/（fg·ml^-1）	实际检测AFB1浓度/（fg·ml^-1）	相对标准偏差/（RSD，%）	回收率/%
0.0	0.00	0.00	100.0
12.5	12.10±0.22	7.04	96.8
50.0	53.20±0.43	12.00	106.0
200.0	206.40±0.95	8.01	103.2