基于荧光法的溶解氧传感器研制及试验

doi:10.12133/j.smartag.2020.2.2.202005-SA004

Smart Agriculture ›› 2020, Vol. 2 ›› Issue (2): 48-58.doi: 10.12133/j.smartag.2020.2.2.202005-SA004

• 专题--农业传感器与物联网 • 上一篇下一篇

基于荧光法的溶解氧传感器研制及试验

顾浩¹, 王志强¹, 吴昊², 蒋永年³, 郭亚¹()

^1.江南大学物联网工程学院/轻工过程先进控制教育部重点实验室，江苏无锡 214122
^2.江苏省互联网农业发展中心，江苏南京 210017
^3.江苏中农物联网科技有限公司，江苏宜兴 214200

收稿日期:2020-05-12 修回日期:2020-06-25 出版日期:2020-06-30
基金项目:
国家自然科学基金(31771680)
作者简介:顾浩（1997－），男，硕士研究生，研究方向为传感器。E-mail：guhao0222@163.com。
通信作者: 郭亚（1977－），男，博士，教授，研究方向为传感器与仪器，系统建模与控制，农业物联网与大数据分析。电话：0510-85910652。E-mail：guoy@jiangnan.edu.cn

A Fluorescence Based Dissolved Oxygen Sensor

GU Hao¹, WANG Zhiqiang¹, WU Hao², JIANG Yongnian³, GUO Ya¹()

^1.School of Internet of Things Engineering, Jiangnan University/Key Laboratory of Advanced Process Control for Light Industry, Ministry of Education, Wuxi 214122, China
^2.Jiangsu Internet Agricultural Development Center, Nanjing 210017, China
^3.Jiangsu Zhongnong Internet of Things Technology Co. , Ltd. , Yixing 214200, China

Received:2020-05-12 Revised:2020-06-25 Online:2020-06-30
Foundation items:National Natural Science Foundation of China(31771680)
About author:Gu Hao, E-mail：guhao0222@163.com
Corresponding author:GUO Ya, E-mail：guoy@jiangnan.edu.cn

摘要/Abstract

摘要：

溶解氧含量的测量对水产养殖具有极其重要的意义，但目前中国市面上的溶解氧传感器存在价格昂贵、不能持续在线测量及更新部件维护困难等问题，难以在水产养殖物联网中大规模推广和发挥作用。本研究基于荧光淬灭原理，利用水中溶解氧浓度与荧光信号相位差的关系进行低成本、易维护溶解氧传感器的研发。首先利用自制备溶氧敏感膜，经激发光照射后产生红色荧光，该荧光寿命可由溶解氧浓度调节；然后利用光信号敏感器件设计光电转化电路实现光信号感知；再以STM32F103微处理器作为主控芯片，编写下位机程序实现激发光脉冲产生，利用相敏检波原理以及快速傅里叶变换（FFT）计算激发光与参照光的相位差，进而转化为溶解氧浓度，实现溶解氧的测量。荧光探测部分与系统主控部分采用分离式设计思想，利用屏蔽排线直接插拔连接，便于传感器探测头的拆卸、更换、维护以及实现远距离在线测量。经测试，本溶解氧传感器的测量范围是0~20 mg/L，响应延迟小于2 s，溶氧敏感膜使用寿命约1年，可以实时不间断地对溶解氧浓度进行测量。同时，本传感器具有测量方便、制作成本低、体积小等特点，为中国水产养殖低成本溶解氧传感器的研发与市场化奠定了良好的基础。

关键词: 溶解氧传感器, 荧光淬灭, 水产养殖, STM32微处理器, 溶氧敏感膜

Abstract:

The measurement of dissolved oxygen content in water is of great significance to aquaculture. However, the dissolved oxygen sensors on the market in China are expensive, and are difficult to maintain continuous online measurement and update parts, so they cannot be widely applied in real production and play expected role in the aquaculture Internet of things(IoT). Based on the principle of fluorescence quenching, a low cost and easy maintenance of dissolved oxygen sensor was developed in this work based on the relationship between the concentration of dissolved oxygen in water and the phase difference of fluorescence signal. The self-made oxygen-sensitive membrane was used to generate red fluorescence which being excited by blue light, and the fluorescence life was regulated by the concentration of dissolved oxygen. Photoelectric conversion circuit with optical signal sensing device was designed to sense optical signal. The STM32F103 microprocessor was used as the main control chip, and the lower computer program was programmed to generate the excitation light pulse. The phase-sensitive detection principle and fast Fourier transform (FFT) were used to calculate the phase difference between the excitation light and the reference light, which was converted into the concentration of dissolved oxygen and realized the measurement of dissolved oxygen. The fluorescence detection part and the main control part of the system were designed as detachable independent modules, and shield lines were used to plug and pull directly, so as to facilitate replacement and maintenance and realize online remote measurement. The testing results showed that, the measurement range of the sensor was 0-20 mg/L, system time delay was less than 2 s, and the life time of the oxygen sensitive membrane would be about 1 year. The dissolved oxygen sensor has the characteristics of convenient measurement, stable result output, low cost and small volume, which will lay a good foundation for the development and marketization of low-cost dissolved oxygen sensors in aquaculture industry of China.

Key words: dissolved oxygen sensor, fluorescence quenching, aquaculture, STM32 microprocessor, oxygen sensitive membrane

中图分类号:

TP212.6

顾浩, 王志强, 吴昊, 蒋永年, 郭亚. 基于荧光法的溶解氧传感器研制及试验[J]. 智慧农业(中英文), 2020, 2(2): 48-58.

GU Hao, WANG Zhiqiang, WU Hao, JIANG Yongnian, GUO Ya. A Fluorescence Based Dissolved Oxygen Sensor[J]. Smart Agriculture, 2020, 2(2): 48-58.

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基于荧光法的溶解氧传感器研制及试验

A Fluorescence Based Dissolved Oxygen Sensor

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